Date of Completion


Document Type

Research Project

Degree Name

Grade 12


ethanolic extract, Anti-angiogenesis, Petroselinum crispum, Chorioallantoic membrane, Methotrexate, Parsley


The study focused on Anti-angiogenesis as one of the standard treatments for various kinds of solid tumor as well as being studied as a manner to stop the development of other angiogenesis-dependent diseases. The researchers used Petroselinum crispum (Parsley) extract extracted from a 10-day dried parsley leaves that will be blended. Stems must first be separated. Blend until the leaves are powderized and placed in an airtight jar. 100% concentrated Ethanol is poured in the jar and was left to set for 3 days until all the degree sinks to the bottom leaving a clean layer of ethanol extract at the top. A clean layer of ethanol extract. A clean layer of ethanol extract at the top. The extract was then obtained through a Rotary Evaporator which now becomes the crude extract which will then undergo a water bath. The extract was computed into 3 concentrations. Concentration 1 consists of 25 ppm, concentration 2 consist of 50 ppm, and the 3rd concentration consisting of 75 ppm. Each was been diluted in a 1000L bottle of distilled water and injected into the air sac of a 10-day old duck egg that serves as the treatment. The negative control will made use of distilled water and the positive control consist of the Methotrexate. The egg was examined on the 4th day of experimentation. The embryo will be placed in a Petri Dish and the last thing to do is count and record the blood vessels. The blood vessels will then be categorized to Primary, Secondary, and Tertiary. Each record was statistically computed to find the optimal number of blood vessels. Furthermore, based from the result of the experiment, the used of Petroselinum crispum (Parsley) was proven to have an anti-angiogenic activity, due to the blood vessel decreasing in every concentration and other control treatment such as Methotrexate however, based from the result, the concentration used shows comparable effect but based from the statistical analysis of the result there is no significant difference between concentration.

First Advisor

James P. Rapanut