Assessment of the antibacterial activity of Basella rubra Linn (Alugbati) seed oil extract on Escherichia coli and Staphylococcus aureus.

Lara Victoria L. Dueñas
Ann Michelle Gosiaco
Angelica Ysabelle S. Soberano
Rozette Angela T. Valdez
Rae Jazmine P. Villaflor

Abstract

The experiment will be conducted in order to assess the antibacterial activity of Alugbati seed oil extract against E. coli and S. aureus. It is known that Alugbati leaf and stem extracts have exhibited antibacterial activity against gram-negative type and gram-positive type of bacteria but it is not yet known whether its seed extract also possesses a possible antibacterial activity. For this study, the oil extract of the seed of the Alugbati plant will be used. Also, the most effective concentration levels of the Alugbati seed extract will be determined. The research design will follow an experimental type of methodology. An analysis of tabulated data will be used as a data gathering tool that will limit the coverage of this study. The data that will be collected during the experiment is objective. This will help broaden the knowledge of whoever may encounter this study and may come to conclusions that may help health practitioners and even those who are not. The study will not cover the difference of the Alugbati seed extract among its leaf and stem extracts. The researchers will utilize two methods which are the Kirby-Bauer test and Broth Macrodilution test. This study will be conducted in a Chemistry Laboratory in De La Salle Medical and Health Sciences Institute or in a laboratory in De La Salle University – Angelo King Medical Research Centre, Dasmarinas, Cavite. The study does not intend to compare the said seed extract to other commercially available antibiotics. The possible side effects of the seed extract will not be tackled as well.