Date of Completion
6-2019
Document Type
Research Project
Degree Name
Grade 12
Keywords
Toxicity Tests, Teratogens, Propylene Glycol, Teratogenic Effect, acute toxicity (FET) Test, zebrafish
Abstract
The experimental method of research was used in the study. The researcher determined the teratogenic effect of the main electronic cigarette component propylene glycol in different concentrations using OECD 236 Zebrafish (Danio rerio) acute toxicity (FET) test. The results showed that the highest concentration of propylene glycol (Treatment 5 (2500ppm)) exhibits the highest teratogenic potential 3dpf inclusive of the four parametric observations such as coagulation of embryo, lack of somite formation, lack of tail detachment, and lack of heartbeat. The LC50 of the treatments was found to be at 977.23 ppm. The treatments showed significant difference with each other in terms of the percentage of dead 3dpf. With regards to this, the concentration of propylene glycol is directly proportional to the percentage of dead embryos. The concentrations of propylene glycol also showed no statistically significant difference with positive control (50% ethanol). This resulted to the moderate toxicity of propylene glycol in zebrafish embryo using the OECD 236 zebrafish (Danio Rerio) acute toxicity (FET) test.
First Advisor
Mark Lawrence F. Fabregas
APA Citation
Princer, M. F., Basa, J. B., Griño, R. S., Lingatong, A. M., & Sazon, D. L. (2019). In vivo analysis on the teratogenicity of the main electronic cigarette component proplylene glycol using OECD 236 zebrafish danio rerio acute toxicity (FET) test. [Research output, De La Salle Medical and Health Sciences Institute]. GreenPrints. https://greenprints.dlshsi.edu.ph/grade_12/13/