Antibacterial activity of Sandoricum koetjape (Santol) leaf ethanolic extract against Staphylococcus aureus and Escherichia coli

Date of Completion


Document Type

Research Project

Degree Name

Grade 12


Antibacterial activity, Escherichia coli, Staphylococcus aureus, Sandoricum koetjape, Santol, leaf extract, ethanolic extract


Sandoricum koetjape, also known as santol, is a tropical fruit belonging to the Meliaceae family. This plant is used to cure diarrhea and fever, and to treat ringworm and armyworm. On the other hand, Staphylococcus aureus is a Gram-positive bacterium that lives in skin, hair, nose, throat and mucous membranes of warm-blooded animals. Its infections are common and may be acquired in hospital settings. Escherichia coli is a Gram-negative bacterium that can be found in the stomach and intestines of humans and warm-blooded animals. Other strains of E. coli can cause diarrhea, abdominal pain, cramping, nausea, and vomiting. In this study, S. koetjape was used to determine its antibacterial property against S. aureus and E. coli. Ethanolic extract of S. koetjape leaves was prepared into three treatment groups with concentration of 25%, 50% and 75% along with the control groups. The groups were subjected with the two bacteria (S. aureus and E. coli) to determine the antibacterial activity of the extract. Answering the statement of the problem number 1, the effect of the extract is the inhibition of growth of S. aureus and E. coli for a certain measure of the zone of inhibition. There is a significant difference between the zone of inhibition which suggests that the inhibitory effect of the various concentrations is different from one another. By comparing the average zone of inhibition for both S. aureus & E. coli, T3 is the most effective treatment among others. This study proved that the Sandoricum koetjape leaf ethanolic extract had an effect against Staphylococcus aureus and Escherichia coli.

First Advisor

Vivian B. Ramirez

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