Antibiogram and Heavy Metal Tolerance Patterns of Pseudomonas Isolates Collected from Imus River Watershed in Cavite, Philippines

Publication Date


Document Type


Publication Title

Turkish Journal of Computer and Mathematics Education


This study was conducted to identify the species of Pseudomonaspresent in the Imus River Wateshed and determine the antibiotic resistance and metal tolerance patterns of Pseudomonasisolates. Water samples were collected from five stations, namely: BaliteBridge (Station 1), Dasmariñas Bridge (Station 2), Palanan Bridge (Station 3), Imus Toll Bridge (Station 4) and Island Cove Bridge (Station 5). The highest total plate count and Pseudomonascount was obtained in Dasmariñas Bridge Station (1.40 x 108 and 9.65 x 107), followed by Palanan Bridge Station (1.92 x 105and 9.7 x 103), Island Cove Balite Bridge Station (1.64 x 104 and4.4 x 102), Bridge Station (1 x 104 and 3.4 x 102) and Imus Toll Bridge Station (5.2 x 103 and 4.01 x 103). The Pseudomonasisolates were identified as P. aeruginosa and P. stutzeri. Antibiotic susceptibility testing revealed that all isolates were found resistant to ampicillin, amoxicillin, cephalexin and erythromycin, and susceptible to streptomycin, ofloxacin, doxycycline and tetracycline. All P. stutzerishowed resistance to clindamycin compared to 77.89% of P. aeruginosaisolates. Twenty-one (22.11%) and 74 (77.89%) isolates of P. aeruginosa exhibited tetra-and penta-drug resistance, respectively while P. stutzeriisolates exhibited penta-drug resistance. Majority of Pseudomonasisolates were resistant to 400 ppm concentration of heavy metals with exception of mercury and cobalt. They were found tolerant to 800 ppm concentration of zinc and arsenic. Penta-and hexa-tolerance of isolates were observed at 400 ppm concentration of heavy metals while bi-and tri-tolerance were recorded on 800 ppm concentration of heavy metals.

First Page


Last Page