Date of Completion


Document Type


Degree Name

Bachelor of Science in Pharmacy


Mayana, Coleus scutellarioides, antioxidants, brine shrimp lethality assay (BSLA), DPPH assay, FRAP assay


Mayana (Coleus scutellarioides) is a common ornamental houseplant in the Philippines, but few studies have been conducted on its medicinal properties. This study aimed to determine the antioxidant mechanisms of methanolic leaf extracts of mayana using various methods. The plant samples were dried and macerated with methanol. Various phytochemical screening tests show that the samples contain flavonoids, anthraquinone glycosides, tannins, and anthocyanins. The brine shrimp assay tested the toxicity of mayana extracts, and determined that a 1000 �g/mL concentration is quite cytotoxic, with the LC50 at 107.29 �g/mL. In the DPPH assay, the highest % inhibition of the samples was 4.36% and 11.10% at 62.5 �g/mL for samples 1 and 2, respectively. The IC50 of sample 2 (272.61 �g/mL) was lower than sample 1 (620.41 �g/mL), indicating that sample 2 has higher antioxidant activity. The highest equivalent FRAP value is 41.53 �mol/L for sample 1 and 50.10 �mol/L for sample 2. Both antioxidant assays indicate that the extracts exhibit dose-dependent activity. From the results, it can be observed that the samples exhibit activity that is comparable to lower concentrations of the standard (Vitamin C). The differences in activity between the samples were all statistically significant, except for some concentrations. The difference in activity between the samples and the standard were also statistically significant.

First Advisor

Alicia P. Catabay