In vitro study on the inhibitory effect of the cell free extract of lactobacillus acidophilus BIOTECH 1900 on alpha glucosidase

Date of Completion


Document Type


Degree Name

Bachelor of Science in Medical Laboratory Science


Lactobacillus acidophilus, alpha-Glucosidases


This study utilized experiential research design which systematically included the determination of the acid tolerance of L. acidophilus, preparation of the cell free extracts lyophilized at UPLB-BIOTECH and the measurement of alpha glucosidase inhibition using microtiter plate reader. Data was analyzed through mean, standard deviation and ANOVA. The results of study revealed that Lactobacillus acidophilus BIOTECH 1900 did not survive at pH 2.5 and at pH 3. The concentration of 0.13 mg/mL had the highest inhibition percentage. A significant difference was noted among the samples as proven by a p-value of <0.05. The study concluded that Lactobacillus acidophilusw BIOTECH 1900 cannot significantly inhibit alpha glucosidase as compared to acarbose.

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