Title

Inhibitory activity of volatile oil of Ocimum basilicum L. (sweet basil) leaves against Mycobacterium tuberculosis

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Mycobacterium tuberculosis, Ocimum basilicum, Antibacterial Agents

Abstract

This study utilized an experimental design which composed of three (3) trials and two (2) replicates. This was consist of one (1) experimental groups and three (3) control groups; the experimental group were conducted with volatile of O. basilicum leaves which will confirm the given hypothesis while the control group were conducted with the Mycobacterium tuberculosis strains (H37Ra, H37Rv and Isoniazid Resistant) and 200 uL of 7H9-S broth as the negative and sterility control. Statistical analysis was obtained upon using receiver operating characteristic curve. The results of the study showed that the H37Ra Mycobacterium tuberculosis were susceptible from 200 ug/mL to 6.25 ug/mL and were resistant from 3.125 ug/mL to 0.390625 ug/mL same with its duplicate, H37Rv Mycobacterium tuberculosis were susceptible from 200 ug/mL to 6.25 ug/mL and were resistant from 6.25 ug/mL to 0.390625 ug/mL and lastly, isoniazid resistant Mycobacterium tuberculosis were susceptible from 200 ug/mL to 12.5 ug/mL and were resistant from 6.25 ug./mL to 0.390625 ug/mL same with its duplicate. Based on the results, the study concluded that the volatile oil of Ocimum basilicum leaves has shown an inhibitory activity against Mycobacterium tuberculosis by having a minimum inhibitory concentration that ranges from 12.5 ug/mL to 6.25 ug/mL.

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