Hepatoprotective activity of Citrus microcarpa (Calamansi) leaf extract against acetaminophen-induced hepatotoxicity in wistar rats

Date of Completion


Document Type


Degree Name

Bachelor of Science in Medical Laboratory Science


Citrus, Rats, Acetaminophen


This research utilized an experimental design wherein the researchers used systematic and scientific approach in collecting and making C. microcarpa (calamansi) leaf extract. Twenty five (25) rats was divided into five groups each consisting of five rats. Group I served as negative control group (NSS). Group II served as the positive control group (silymarin) while the groups III, IV, and V were administered with C. microcarpa leaf extract at doses of 0.01 g/kg, 0.05 g/kg, and 0.1 g/kg respectively. After one month of treatment, 500 mg/kg of acetaminophen was given to all groups of rats to induce damage. The researchers evaluated the Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) levels as well as the histopathological examination to assess the liver of the Wistar rats. The results of the study showed that the C. microcarpa leaf extract showed a significant effect after one month treatment. The ALT levels of Wistar rats induced with acetaminophen was significantly reduced (p<0.05) upon treatment of C. microcarpa leaf extract for one month of minimum concentration of 0.01 mg/kg. However, there was no significant difference between the three concentrations of the extract in decreasing the ALP levels. The extract was also capable of preventing visible cell damage and lesions in the liver and a significant difference between silymarin and C. micocarpa leaf extract was observed (p<0.05). The study concluded that the C. microcarpa leaf extract with different concentrations is capable of lowering the ALT and ALP levels of acetaminophen-induced hepatotoxicity in Wistar rats after one month of treatment and showed no visible cell damage in the liver.

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