Title

Inhibitory activity of cell-free extract of Saccharopolyspora arythraea against Citrobacter koseri and Stenotrophomonas maltophilia

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Saccharopolyspora, Citrobacter koseri, Stenotrophomonas maltophilia

Abstract

The study utilized an experimental research design using Kirby Bauer disk diffusion assay to determine the inhibitory activity of Saccharopolyspora erythraea against Citrobacter koseri and Stenotrophomonas maltophilia. Serial dilution was used to determine the minimum inhibitory concentration. The minimum bactericidal concentration was determined by sub-culturing clear samples on treated test tubes from MIC and counting the growth of colonies. The result of the study showed that the cell-free extract of Saccharopolyspora erythraea showed no inhibitory activity against Stenotrophomonas meltophilia. However, the cell-free extract showed 14-16 mm zone of inhibition against Citrobacter koseri compared to the Piperacillin (positive control) which have 32-35 mm, 3, 125v/v% and 100v/v% of the minimum inhibitory concentration and 3.125v/v% and 100v/v% minimum bactericidal concentration for C. koseri and s. maltophilia respectively, was determined. The quantified macrolid using spectrophotometry was 0.9367 mg/mL. The study concluded that the cell-free extract of Sacchropolyspora erythrae showed positive inhibitory activity against Citrobacter koseri but has showed no inhibitory activity against stenotrophomonas maltophilia. Therefore, other Saccharopolyspora spp. or other bacterial extract must be tried to stenotrophomonas maltophilia.

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