Anti-biofilm properties of Cananga odorata (ylang-ylang) essential oil against Pseudomonas aeruginosa

Date of Completion


Document Type


Degree Name

Bachelor of Science in Medical Laboratory Science


Biofilms, Pseudomonas aeruginosa, Cananga


The study utilized completely randomized experimental research design in assessing the anti-biofilm properties of C. odorata essential oil against P. aeruginosa. Biofilm produced by P. aeruginosa were confirmed using Crystal Violet Assay. Minimum Biofilm Inhibitory Concentration (MBIC) and Minimum Inhibitory Concentration (MIC) determined to assess whether the biofilm and growth formation of the P. aeruginosa were inhibited. Scanning Electron Microscope (SEM) was used in order to visualize if the biofilm formation of P. aeruginosa was disrupted. Based on the results, significant inhibitory effects of C. odorata essential oil was observed against the biofilm formed by P. aeruginosa. Biofilm formed by P. aeruginosa on the microtiter plate has been verified by Crystal Violet Assay. Images of P. aeruginosa biofilm with the highest concentration of the ylang-ylang essential oil on 2000x magnification shown disruption of the biofilm. The study concluded that the use of C. odorata essential oil as an anti-biofilm agent has been shown using various concentrations of the extract applied to P. aeruginosa cultures on microtiter plates and disruption of the biofilm determined. The MBIC and MIC was shown to be at 50% concentration of C. odorata essential oil.

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