Title

Neutralizing property of Bryophyllum pinnatum (Katakataka) and Eleusine indica (Paragis) leaves extract against Naja philippinensis venom

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Kalanchoe, Eleusine, Naja

Abstract

Indirect hemolytic assay was used in vitro testing, wherein in 30 ul of venon was applied to the wells on agarose-erythrocyte-egg yolk plate and were incubated overnight at 37°C and produced a hemolytic halo, after which different treatment concentrations of Bryophyllum pinnatum and Eleusine indica crude extracts were used in increasing order (10, 30, 50, 70 and 100 ug/mL). In vivo phase include the administration of the extracts on Eudrilus euginae (10, 30, 50, 70 and 100 ug/mL). In vivo phase include the administration of the extracts on Eudrilus euginae (African Night crawler) wherein at their posterior end and its motor skills were observed for 24 hours, with monitoring interval of 30 minutes. After observation, the living and the dead earthworms were counted. Results of the study showed that phytochemical testing shows that Bryophyllum pinnatum leaves extracts has flavonoid (quercetin) while Eleusine indica leaves extracts has phenol (gallic acid). Indirect hemolytic assay results shows both crude extracts reduced the hemolytic halo of the venom. Bryophyllum pinnatum and Eleusine indica exhibit neutralizing property in which the length of survival of earthworms varies on the crude extract concentration that is monitored for 24 hours. The study conclude that there is a significant difference between Bryophyllum pinnatum leaves extracts and commercially available antivenom on 10, 30, 50, 70 mg/dl concentrations while the 100 mg/dl has no significant difference. While the Eleusine indica leaves extract and commercially available antivenom has significant difference on 10, 70 and 100 mg/dl concentration while in 30 and 50 mg/dl which has no significance difference.

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