Anti-angiogenic activity of the ethanolic extract of Cocus nucifera (coconut) husk using chorioallantoic membrane (CAM) assay

Date of Completion


Document Type


Degree Name

Bachelor of Science in Medical Laboratory Science


Cocos, Ethanol, Angiogenesis Inhibitors, Allantois


The researchers utilized an experimental research design. One hundred and twenty (120) duck eggs were classified into five set-ups with eight eggs per group and were introduced with phosphate buffered saline, 50 mg/mL, 75 mg/mL, and 100 mg/mL of nucifera hust ethanolic extract, and 25 mg/mL methotrexate. The phytochemical screening of the ethanolic extract was done at the University of the Philippines Manila. Angiogenesis after the treatment introduction was quantified through counting the number of blood vessels using WimCAM tool by Wimasis Image Analysis. The study concluded that all concentrations of the C. nucifera hust (50mg/mL, 75 mg/mL and 100 mg/mL) extract successfully inhibited vascular formation and proliferation in varying degrees on the CAM assay wherein 100 mg/mL C. nucefera came out as the optimal concentration for anti-angiogenic activity.

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