Title

Agglutinating property of of Abelmoschus esculentus (Okra) and Cucumis sativus (Cucumber) for rapid identification of Vibrio cholerae

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Abelmoschus, Cucumis sativus, Cholera

Abstract

Experimental research design was used in this study. Mixtures of the protein crude extract and V. cholerae was done in microtiter plates for agglutination reaction and assessed by reading the optical density using microtiter plate reader at 655 nm and further confirmed by microscopic analysis for the presence or absence of agglutination. The results showed that Cucumis sativus (cucumber) exhibit greater agglutinating activity than Abelmoschus esculentus (okra). It shows that not all formulated concentrations for A. esculentus does not decrease its optical density. And further examination under microscope shows that Cucumis sativus (cucumber) presence of agglutination and in contrast absence of agglutination in Abelmoschus esculentus (okra). The study concluded that both of the crude extracts have a significant difference by comparing their calculated mean in terms in the change of absorbance under the wavelength of 655 nm and based on the microscopic analysis results Cucumis sativus (cucumber) is the crude extract that exhibited agglutinating activity against Vibrio cholera.

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