Title

Antibiofilm activity of chlorogenic acid extracted from green coffee (Coffea liberica) beans against acinetobacter baumannii

Date of Completion

4-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Antibiofilm, chlorogenic acid, Coffea liberica, coffee beans, acinetobacter baumannii

Abstract

The study used an experimental and quantitative design to assess the antibiofilm properties of chlorogenic acid extract against A. baumannii. Biofilm formed by A. baumannii were determined using Crystal Violet Assay. Minimum Inhibitory Concentration (MIC) and Minimum Biofilm Inhibitory Concentration (MBIC) were determined through Microdilution Procedure and Crystal Violet Assay to assess whether the growth and biofilm formation of A. baumannii after 24 hours of incubation. Scanning Electron Microscopy (SEM) was used to confirm and visualize if the biofilm formation of A. baumanni was disrupted. Biofilm formed by A. baumannii on the microtiter plate has been verified through Cystal Violet Assay. The MIC was found to be at 6.25% concentration of chlorogenic acid extract. MBIC and MBEC was found to be at 0.195% concentration of chlorogenic acid extract. Images of A. baumanni biofilm with the highest concentration of chlorogenic acid extract of 3000x magnification showed distruption of the biofilm. The study concluded that chlorogenic acid extracted from green coffee beans has a potential antibiofilm activity against a. baumanni. There is a significant difference between the commercially available antibiotics and chlorogenic acid extracted from green coffee beans.

First Advisor

Jion P. Dimson, RMT

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