Title

Efficacy of Benzalkonium chloride against biofilm-embedded pseudomonas aeruginosa

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Benzalkonium Compounds, Pseudomonas aeruginosa

Abstract

This study used experimental research design. Tryptic soy broth and agar were used for the propagation of bacteria. A 96-polyvinylchloride (PVC) flat-bottomed well plates were used to screen for the formation of P. aeruginosa biofilm and to test the microbial activity of antiseptics in various concentrations (Djordjevic, et al., 2002). Phosphate-buffered saline (PBS) with a pH of 7.4 was used a s buffer to wash the preformed biofilms. Crystal violet, a dye that binds to proteins and DNA and is a simple method to detect maintained adherence of cells (Feoktistova, et al. 2016), was used for the staining of bacteria slimes. The micro titer plate will be submerged in a tub full of water, which help in removing unattached cells and media components that can be stained (Abdi-ali, et al., 2014). Statistical analysis used were mean, percent reduction, One-way ANOVA and Post-Hoc Analysis Tukey's (HSD). The study concluded that pseudomonas aeruginosa showed resistance when subjected to lower concentration of benzalkonium chloride at a shorter contact time but exhibited inhibition to higher concentration at a longer contact time.

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