Title

Inhibitory capabilities of farnesol with nisin against candida albicans

Date of Completion

2017

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Farnesol, Nisin, Candida albicans

Abstract

This study used experimental research design. Twenty isolates of Candida albicans are treated with different concentrations of separated farnesol and nisin to assess its MIC. Prior to determining the synergism of both antimicrobials, Checkerboard Assay with some modification was used. The results of the study showed that the concentration used for farnesol was 22.24 ug/ml to 72.27 ug/ml and candida albicans was partically inhibited at 72.27 ug/ml. While for nisin, the range of the concentrations used are from 300 to 600 ug/ml and candida albicans was partically inhibited at 350 ug/mL. The true MICs for the clinical isolates needed higher concentrations of inhibitory compounds compared to the pure isolates based on related literature. The study concluded that the MIC of farnesol can be further obtained by using higher concentrations than the ones used in this study. Due to farnesol's poor solubility, it is used at low concentrations. However, the concentrations were not able to fully inhibit the growth of Candida albicans. It is proven that by adding nisin, efficacy of farnesol can be enhanced.

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