Date of Completion

2024

Document Type

Thesis

Degree Name

Bachelor of Science in Medical Laboratory Science

Keywords

Amoebiasis, Entamoeba histolytica, animal model, Sprague-Dawley rats, amoebic liver abscess

Abstract

Amoebiasis is a commonly acquired parasitic infection among humans that is predominantly caused by the protozoa Entamoeba histolytica. Symptoms of the disease include the extent of infection in the gastrointestinal tract which may extend its severity in the form of extraintestinal infections. This study aims to establish the capability to contrive an in vivo acculturation and an animal model which aims to proportionately simulate an infection in a nonhuman organism; ultimately, the study incorporated the amebiasis infection to Sprague-Dawley rats, which are the chosen mammalian organisms to simulate the clinical presentations of amebiasis. The study proposed that the confirmative development of amoebic liver abscess with complement of stools indicating amoebic infection implies the susceptibility. Standardized rats in an environmentally controlled condition were inoculated with an Entamoeba histolytica load by oral gavage and were incubated and allowed for the development of infection for 10 days; a negative control group was also subjected to oral gavage with normal saline solution. Consequently, none of those belonging to the experimental group were able to present signs of amoebic infection based on fecal dispensation due to the absence of watery stool consistency and absence of cyst forms under low power objective microscopy, while two (2) out of the ten (10) rats developed non-amebic liver abscess. The results of the study definitively imply mere susceptibility of the rats to amoebic infection. Furthermore, the study sets forth underpinnings for future research in establishing a more standardized and statistically sound animal model for simulation and clinical trial use.

Download

Share

COinS