Date of Completion


Document Type


Degree Name

Bachelor of Science in Biochemistry


Enterobacteriaceae, Vibrio cholerae, coliform, Labac Riever, Water quality


The Philippines is an archipelago teeming with natural resources and bodies of water. The major source of freshwater came from lakes, swamps, rivers, reservoirs and groundwater. The waste materials from residential areas and commercial buildings tantamount to the accumulation of toxic chemical and diseases from infectious organisms such as as cholera, which caused by infectious, gram -negative, and motile bacteria call Vibrio cholerae. The physicochemical parameters of the water samples from the Labac River in Naic Station conducted in this study are color, ligh absorbance, light penetration, temperature, totalsuspended solids (TSS), pH, total alkalinity, total dissolved solids (TDS), hardness, biological oxygen demand (BOD), dissolved oxygen (DO), phosphorus, chloride and magnesium determination. Total coliform and biochemical analysis were also performed which included indole test, citrate utilization test, urease test, voges proskaur test, and methyl red test. Majority of the samples exhibited class C water quality and high contents of coliform bacteria except substation 1. The results from the biochemical testing suggested that the identity of the cultured bacteria is presumably Vibrio cholerae though further testing should be applied for detercting specific strains. For the analysis of variance, it is shown that majority the of values for the physicochemical parameters are less than 0.0001 excpt for light absorbance which has 0.428. The overall quality of water in Labac River Naic Station met the standard for class C water.

First Advisor

Iluminada A. Ronio, LPT, MSc