Title

In vivo antipyretic and analgesic effects of the ethanolic extract of Bauhinia malabarica leaves in ICR albino mice

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Analgesics.

Abstract

The increasing emergency room visits of about 421 for every 1,000 individuals in the population and the adverse effects associated with taking synthetic antipyretic or analgesic drugs. The most common reasons for ER visits were fever, chest pain and abdominal pain. The ethanolic extract of B. malabarica was subjected to phytochemical screening before in vivo assays. It was administered to male Institute of Cancer Research (ICR) albino mice in 700 mg/kg, 850 mg/kg and 1000 mg/kg doses. The mice were subjected to antipyretic and analgesic screening methods wherein the percent reduction in temperature and percent increase in reaction time are expressions of the antipyretic and analgesic effects. Phytochemical analysis revealed the presence of polyphenolic flavonoids, sterols and tannins. A decrease in the tail temperature after 3 hours indicated antipyretic effect as observed in the standard antipyretic paracetamol (25.77±0.07) and an increase in reaction time after 3 hours indicated analgesic effect in the standard analgesis aspirin (33.97±0.94) to which all the different doses of the extract were compared. Results revealed that all three doses decreased the tail temperature and increased the reaction time of mice in both tests as compared to the control (p<0.01). However, only the 700 mg/kg dose (25.90±0.06 in YPT and 36.14±3.14 in HPT) was sustained and comparable to the standard drugs. Thus, the antipyretic and analgesis effects of the extract were not dependent on the doses used since the highest concentration of the extract was less beneficial. However, further studies on the isolation and purification of the active compounds in B. malabarica ethanolic extract are necessary in order to support the antipyretic and analgesic effects observed in this study.

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