Title

In vitro screening of antibacterial activity of selected Philippine artocarpus species

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Anti-Bacterial Agents.

Abstract

Many plants have been known for their beneficial usis in curing infectious diseases and illnesses. The extracts and metabolites of Artocarpus have bioactive compounds that can be used in antbacterial, antifungal and antiviral activities. The present study investigated the phytochemical and antibacterial properties of the leaf extracts from selected Philippine Artocarpus species: Artocarpus camansi, Artocarpus nitidus and Artocarpus blancoi. Hexane, dichloremethane and methanol were used as extraction solvents and Escherichia coli and Staphylococcus Aureus , were used as test organisms. A Kirby-Bauer method was adopted to test the susceptibility of the selected microorganisms to the plant extracts and specific antibiotics. Minimum inhibitory concentration (MIC) was determined using serial dilution of plant extracts. Phytochemical screening by thin-layer chromatography was carried out by specific chemical identification tests for characterization of crude extracts. Bioassay data were statistically analyzed using one-way ANOVA (SPSS at 95% confidence level). Methanolic extract of A. camansi and A. nitidus possessed significant antibacterial activity against S. aureus with s14.00 ± 0.35 mm and 13.40 ± 0.35 mm inhibition zone radii respectively. Dichloromethane and hexane extracts of leaf showed no antibacterial activity against both test organisms. Bacterial antibiotic doxycycline and chloramphenicol whcih were used as positive controls were more effective against E. coli and S. aureus for methanolic extracts. Phytochemical screening confirmed the presence of flavonoids, steroids, sterols, alkaloids, sugars, anthraquinones, anthrones, courmarins, indoles, higher alcohols, essential oils, tannins and phenolic compounds in leaf extracts. These results confirm the potential antibacterial activity of A. Camansi and A. nitidus and the presence of medicinally important phytochemicals in the plants.

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