Title

Biosynthesis of silver nanoparticles using Premna odorata (Alagaw) and Saccharum spontaneum (Talahib) leaves and their larvicidal activity against aed

Date of Completion

2018

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Nanoparticles.

Abstract

Copper nanoparticles have been an interesting field due to its wide variety of applications and low cost. This study aimed to synthesize copper nanoparticles from the aqueous stem extract of Ipornea aquatica and to detect its antibacterial properties against E. coli and S. aureus. The aqueous extract was used to reduce the Cu2+ to Cu0 and form the nanoparticles. The reaction was monitored using UV-Vis spectroscopy with the max around 770 nm. The FTR spectra of both raw plant and the synthesized copper nanoparticles showed peaks pertaining to carbonyl, C-O and C-N bonds indicating that biomolecules present in the stem extract are capable of reducing the Cu2+. The size of the synthesized nanoparticles fall within the range 4-5nm. The antibacterial activity of the synthesized nanoparticle showed significant specific activity for gram negative bacteria as compared to gram positive bacteria. Thus this study was able to prove a basis for the antibacterial activity of copper nanoparticles.

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