Date of Completion

7-2019

Document Type

Thesis

Degree Name

Bachelor of Science in Biochemistry

Keywords

Green synthesized nanoparticles, Ipomoea aquatica, iron oxide

Abstract

A number of severe cases due to bacterial infections were reported in the past few years and thus different types of materials have been studied for their antibacterial properties. Green synthesized nanoparticles have been a interesting material for the biomedical field because it is eco-friendly, cost effective and less toxic to human body compared to commercially available drugs. In this study, Ipomoea aquatica leaves aqueous extract and FeCl3 solution were used to prepare green synthesized iron oxide nanoparticles. Absorption peak, morphological structure, sice, chemical components of the nanoparticles were characterized using UV-Vis Spectroscopy, Fourier-transform infrared spectroscopy and Scanning Electron Microscopy (SEM). UV-Vis spectral revealed an absorption peak at 326 nm. Several IR stretching vibrations for the functional groups such as O-H, C=C, C-H, C-O were observed in the iron oxide nanoparticles. The synthesized iron oxide nanoparticles were successfully stabilized with sizes varying from 30 nm-90 nm. The antibacterial assay was conducted against gram positive-Staphylococcus aureus and gram negative-Escherichia coli with agar well diffusion method using Muellar Hinton agar plates. The zone of inhibition was found to be 17.82 mm and 19.68 mm for S. aureus and E.coli respectively. thus, the data collected from this study concluded that green synthesized iron oxide nano particles was cost effective and antibacterial property of FeONPs falls under category of susceptible against gram positive and gram negative bacteria.

First Advisor

Eduardo B. Tibayan

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